The green fluorescent protein has gained significant attention in biology, medicine and research and has been described as the microscope of the twenty first century for a very good reason. Through this protein, it has become easy to not only observe proteins as they are being made, but also observe any movements. By attaching the gene of this protein to the gene of a given protein or an organism, scientists and researchers can easily observe any protein of interest given that GFP fluoresces. Because it can be attached to other proteins and organisms, GFP has become one of the most popular imaging tools.
Green fluorescent protein : properties, applications, and protocols
Green Fluorescent Protein GFP is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the nm laser line and is optimally detected at nm. We offer a series of GFP Invitrogen CellLight fusion constructs of signal peptides or cell structure proteins with emGFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments. To complement these tools, we also offer anti-GFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. Invitrogen CellLight reagents are ready-to-use fluorescent protein constructs targeted to specific subcellular structures.
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